Zebrafish Egg Infection Model for Studying Candida albicans Adhesion Factors

Yin-Zhi Chen, Yun-Liang Yang, Wen-Li Chu, May-Su You, Hsiu-Jung Lo

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Disseminated candidiasis is associated with 30-40% mortality in severely immunocompromised patients. Among the causal agents, Candida albicans is the dominant one. Various animal models have been developed for investigating gene functions in C. albicans. Zebrafish injection models have increasingly been applied in elucidating C. albicans pathogenesis because of the conserved immunity, prolific fecundity of the zebrafish and the low costs of care systems. In this study, we established a simple, noninvasive zebrafish egg bath infection model, defined its optimal conditions, and evaluated the model with various C. albicans mutant strains. The deletion of SAP6 did not have significant effect on the virulence. By contrast, the deletion of BCR1, CPH1, EFG1, or TEC1 significantly reduced the virulence under current conditions. Furthermore, all embryos survived when co-incubated with bcr1/bcr1, cph1/cph1 efg1/efg1, efg1/efg1, or tec1/tec1 mutant cells. The results indicated that our novel zebrafish model is time-saving and cost effective.
原文English
期刊PLoS ONE
10
發行號11
DOIs
出版狀態Published - 16 十一月 2015

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