Widefield multiphoton microscopy with image-based adaptive optics

C. Y. Chang*, L. C. Cheng, H. W. Su, W. C. Yen, Shean-Jen Chen

*Corresponding author for this work

研究成果: Conference contribution同行評審


Unlike conventional multiphoton microscopy according to pixel by pixel point scanning, a widefield multiphoton microscope based on spatiotemporal focusing has been developed to provide fast optical sectioning images at a frame rate over 100 Hz. In order to overcome the aberrations of the widefield multiphoton microscope and the wavefront distortion from turbid biospecimens, an image-based adaptive optics system (AOS) was integrated. The feedback control signal of the AOS was acquired according to locally maximize image intensity, which were provided by the widefield multiphoton excited microscope, by using a hill climbing algorithm. Then, the control signal was utilized to drive a deformable mirror in such a way as to eliminate the aberration and distortion. A R6G-doped PMMA thin film is also increased by 3.7-fold. Furthermore, the TPEF image quality of 1 μm fluorescent beads sealed in agarose gel at different depths is improved.

主出版物標題Unconventional Imaging and Wavefront Sensing 2012
出版狀態Published - 1 十二月 2012
事件Unconventional Imaging and Wavefront Sensing 2012 - San Diego, CA, United States
持續時間: 13 八月 201214 八月 2012


名字Proceedings of SPIE - The International Society for Optical Engineering


ConferenceUnconventional Imaging and Wavefront Sensing 2012
國家United States
城市San Diego, CA

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