In this investigation, we propose and investigate the standard WiMAX access in high speed railway (HSR) using radio over fiber (RoF) link. We also discuss the theory of mobile WiMAX at a target speed of 300 km/hr in simulation. We also analyze the effect of the Doppler frequency shift over HSR. The effective transmission fiber length in the WiMAX-RoF system was limited due to the time-division-duplex (TDD) protocol in the practical WiMAX. Thus, we also demonstrate the transmission limitation and performance of the standard WiMAX access in RoF link. The performances of throughput and packet loss at different fiber lengths are also investigated and discussed. Furthermore, a WiMAX-RoF field trial is also demonstrated based on a simple two RAUs RoF architecture. It was tested and measured in the SongShan tunnel of the Taiwan high speed railway (THSR).