Synthesis of activity probes for β-xylosidase

Lee Chiang Lo*, Chi Yuan Chu, Fang An Lin, Yen Ru Pan, Yaw-Kuen Li, Jing Jer Lin

*Corresponding author for this work

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)

摘要

Due to their industrial interest and significance, developing a rapid method for screening β-xylosidase activities would certainly facilitate the research and improve production in xylan-related industries especially in the pulp and paper industries. Here we report the synthesis of two activity probes, LCL-6X and -12X, for β-xylosidase. They both carry a β-xylopyranosyl recognition head, which is linked to a latent trapping device consisting of a 2-fluoromethylphenoxyl group and a biotin reporter group. They differ only in the length of their hydrophobic linker. A model study using a β-xylosidase from T. koningii demonstrates that both probes could successfully label the target hydrolase and give rise to biotinylated proteins. They could thus potentially become a powerful tool in screening β-xylosidase from microbial sources.

原文English
頁(從 - 到)479-488
頁數10
期刊Journal of the Chinese Chemical Society
53
發行號2
DOIs
出版狀態Published - 1 一月 2006

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