Structural stability of heterologous genes cloned in Streptomyces plasmid pIJ702

Yan-Hwa Wu Lee*, Zee Yuan Tzecheng, Shiang Ching Wang, Wen Ling Cheng, Carton W. Chen

*Corresponding author for this work

研究成果: Article

14 引文 斯高帕斯(Scopus)

摘要

A recombinant plasmid pWCL1 containing Streptomyces plasmid pIJ702, E. coli plasmid pUC12, and hepatitis B viral surface antigen (HBsAg) gene was stably maintained in E. coli, but exhibited structural instability in S. lividans 1326. The deletions were found ranging from 2.75 to 5.65 kilobases (kb) and most of them occurred within the melanin (mel) gene of pIJ702, resulting in the loss of part of the mel gene sequence plus the insert. The removal of the pUC12 sequence from pWCL1 eliminated the instability. However, pUC12 alone inserted in either orientation on pIJ702 also caused the deletion in S. lividans 1326. The results indicated that the structural instability of hybrid plasmid of pIJ702 depended on the interaction between the mel sequence and the inserted sequence.

原文English
頁(從 - 到)372-378
頁數7
期刊Biochemical and Biophysical Research Communications
140
發行號1
DOIs
出版狀態Published - 15 十月 1986

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