Structural and biochemical evidence of mitochondrial depletion in pigs with hypertrophic cardiomyopathy

Chih-Sheng Lin, Y. L. Sun, C. Y. Liu*

*Corresponding author for this work

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Pig hearts with naturally occurring hypertrophic cardiomyopathy (HCM) were isolated to investigate the effects of mitochondrial deficiency at biochemical and molecular levels. Enzyme activities of mitochondrial-encoded cytochrome c oxidase and NADH dehydrogenase in the HCM hearts (n = 12) were lower than that in the controls (n = 12) by 41 ± 29% (P < 0.01) and 43 ± 21% (P < 0.001), respectively. Additionally, Southern blot analysis was conducted to quantify the relative amount of mitochondrial DNA (mtDNA) from the HCM and controls. The relative amount of mtDNA in the HCM hearts was significantly 57 ± 19% (P < 0.001) lower than that in the controls. Both mitochondrial enzyme deficiency and mtDNA depletion were significantly correlated with the degree of cardiac hypertrophy judged based on the ratio of heart/body weight. In conclusion, our results reveal that a secondary effect of tissue-specific mtDNA depletion and mitochondrial dysfunction is in response to the HCM.

原文English
頁(從 - 到)219-226
頁數8
期刊Research in Veterinary Science
74
發行號3
DOIs
出版狀態Published - 1 一月 2003

指紋 深入研究「Structural and biochemical evidence of mitochondrial depletion in pigs with hypertrophic cardiomyopathy」主題。共同形成了獨特的指紋。

引用此