Protein plasticity: A single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien- 3β-ol, a rearrangement product

Tung-Kung Wu*, Hao Yu Wen, Cheng Hsiang Chang, Yuan Ting Liu

*Corresponding author for this work

研究成果: Article

24 引文 斯高帕斯(Scopus)

摘要

(Chemical Equation Presented) To provide insights into the structure-function relationships of oxidosqualene-lanosterol cyclase (ERG7) from Saccharomyces cerevisiae, the Phe699 was exchanged against hydrophilic polar uncharged residues Ser, Thr, Cys, Gln, and Tyr to characterize its product profile and functional role in ERG7 activity. Among the substitutions, only the ERG7F699T mutant produced novel protosta-13(17),24-dien-3β-ol as the sole truncated rearrangement product. The results suggest that Phe699Thr mutation is likely to affect the C-17 cation stabilization during the rearrangement process.

原文English
頁(從 - 到)2529-2532
頁數4
期刊Organic Letters
10
發行號12
DOIs
出版狀態Published - 1 十二月 2008

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