Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase

Yuan Ting Liu, Tain Chang Hu, Cheng Hsiang Chang, Wen Shiang Shie, Tung-Kung Wu*

*Corresponding author for this work

研究成果: Article同行評審

8 引文 斯高帕斯(Scopus)

摘要

A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7T384Y/Q450H/V454I, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.

原文English
頁(從 - 到)5222-5225
頁數4
期刊Organic Letters
14
發行號20
DOIs
出版狀態Published - 19 十月 2012

指紋 深入研究「Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase」主題。共同形成了獨特的指紋。

引用此