Programmable protein crystallization in metered encapsulated droplets

Y. W. Hsu, Chiun-Hsun Chen, S. K. Fan*

*Corresponding author for this work

研究成果: Conference contribution同行評審

摘要

We report for the first time programmable protein crystallization based on tunable evaporation and dissolution of salt and protein precipitants of an aqueous core droplet encapsulated in an oil shell. The evaporation depended on the precisely metered aqueous and oil droplet volumes and the position of the aqueous core droplet. The dissolution of the precipitants was achieved by adjusting the temperature of the aqueous core droplet. Lysozyme protein crystal was obtained within 80 min through (1) rapid salt/protein precipitation by fast evaporation, (2) salt dissolution by heating to enhance protein crystallization, and (3) protein crystallization from the protein precipitants (seeds) by slow evaporation.

原文English
主出版物標題2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
頁面1093-1096
頁數4
DOIs
出版狀態Published - 7 五月 2012
事件2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
持續時間: 29 一月 20122 二月 2012

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Conference

Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
國家France
城市Paris
期間29/01/122/02/12

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