Peak crossover in high-performance liquid chromatography elution monitored using whole-column detection

Shu Hui Lin, Tiing Yu*, Alf Sheu, Der Jyh Yang, Su Cheng Pai

*Corresponding author for this work

研究成果: Article同行評審

5 引文 斯高帕斯(Scopus)

摘要

A whole-column detection (WCD) system was fabricated using the parts from a typical A4 size optical scanner. Optimum layout of the fluorescence tube, beam condenser and photon transducers from the scanner made an effective devise to examine the dynamic chromatographic separation process. This system was applied in monitoring peak crossover in an ion-pair chromatographic elution. A stage-wise gradient was conducted in eluting a mixture of New Coccine and Carmoisine. Compound New Coccine lagging behind after sample injection moved passing Carmoisine at the column outlet. The complete on-column elution process could be observed directly using this detection system. This inexpensive and rugged WCD system provided a 0.3 mm spatial resolution and a 3.6 ms temporal resolution and was proven to be an adequate device to directly monitor solute retention behavior in the liquid chromatography column.

原文English
頁(從 - 到)128-131
頁數4
期刊Journal of Chromatography A
1201
發行號1
DOIs
出版狀態Published - 1 八月 2008

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