Overcoming Multidrug Resistance of Breast Cancer Cells by the Micellar Drug Carriers of mPEG-PCL-graft-cellulose

Yung Tsung Chen, Chao Hsuan Chen, Ming Fa Hsieh, Ann Shireen Chan, Ian Liau, Wan Yu Tai

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

The amphiphilic block copolymers methoxypoly (ethylene glycol)-poly (å-caprolactone) (mPEG-PCL) was grafted to 2-hydroxyethyl cellulose (HEC) to produce watersoluble copolymers. Doxorubicin (DOX)-loaded nanoparticles were prepared by dialysis method and the sizes of nanoparticles were determined by dynamic light scattering (DLS) in solution. The size of the nanoparticles was in the range of 197.4 to 340.7 nm. Rhodamine 123 was used to probe the relative Pglycoprotein (P-gp) expression in human breast cancer cell lines MCF-7/WT and MCF-7/ADR. Confocal laser scanning microscopy (CLSM) showed a difference between the fluorescence images of DOX-loaded micelles and free DOX. For a quantitative basis, flow cytometry was done on both cell lines treated with DOX-loaded micelles and free DOX to compare a difference in effect. The amount of DOX-loaded micelles in MCF-7/ADR increased compared to that in the MCF-7/WT cells. This gives insight into how the micellar system developed in this study overcomes the multidrug resistance (MDR) effect.

原文English
主出版物標題13th International Conference on Biomedical Engineering - ICBME 2008
頁面1224-1227
頁數4
DOIs
出版狀態Published - 1 十二月 2009
事件13th International Conference on Biomedical Engineering, ICBME 2008 - , Singapore
持續時間: 3 十二月 20086 十二月 2008

出版系列

名字IFMBE Proceedings
23
ISSN(列印)1680-0737

Conference

Conference13th International Conference on Biomedical Engineering, ICBME 2008
國家Singapore
期間3/12/086/12/08

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