Observing the dynamic variation of the binding force between rhodostomin ligand and integrin alpha (IIb) beta (3) receptor using a photonic force microscope

Bo Jui Chang*, Chia Fen Hsieh, Chi Hung Lin, Sien Chi, Long Hsu

*Corresponding author for this work

研究成果: Conference article同行評審

摘要

The conformational change of integrin α IIb β 3 plays an important role in clot formation. However, the correlation between the structure and the function of integrin α IIb β 3 in interacting with its ligand is still not clear. In this report, we focus on the dynamic variation of the binding between integrin α IIb β 3 and its ligand, rhodostomin by using a photonic force microscopy (PFM). The PFM is used to trap a rhodostomin-coated bead and, then, shift it to bind a surrounding CHO α IIb β 3 cell. Meanwhile, it tracks, with a resolution of 1MHz, the Brownian fluctuations of the trapped bead Theoretically, the smaller the amplitude of the Brownian fluctuations, the stronger the stiffness of the binding force between the rhodostomin and the CHO α IIb β 3 cell. Experimentally, a significant decrease of the Brownian fluctuations was observed during the interval between the 360 th seconds and the 400 th seconds after the trapped rhodostomin-coated bead contacted an integrin-expressed CHO α IIb β 3 cell. This observation reveals that it takes the rhodostomin 360 seconds to seek the correct position to bind to the integrin α IIb β 3 . After 400 seconds, the rhodostomin has bound rigidly with the integrin α IIb β 3 . We presume that the integrin α IIb β 3 has reached its final stage of conformational change.

原文English
文章編號75
頁(從 - 到)552-559
頁數8
期刊Proceedings of SPIE - The International Society for Optical Engineering
5514
DOIs
出版狀態Published - 1 十二月 2004
事件Optical Trapping and Optical Micromanipulation - Denver, CO, United States
持續時間: 2 八月 20046 八月 2004

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