Murine renal transcriptome profiles upon leptospiral infection: Implications for chronic kidney diseases

Li Fang Chou, Ting-Wen Chen, Huang Yu Yang, Ming Yang Chang, Shen Hsing Hsu, Chung Ying Tsai, Yi Ching Ko, Chiung Tseng Huang, Ya Chung Tian, Cheng Chieh Hung, Chih Wei Yang*

*Corresponding author for this work

研究成果: Article同行評審

7 引文 斯高帕斯(Scopus)

摘要

Background Leptospirosis caused by pathogenic Leptospira spp. leads to kidney damage that may progress to chronic kidney disease. However, how leptospiral infections-induced renal damage is unclear. Methods We apply microarray and RNA-seq technologies to investigate the first murine transcriptome-wide, leptospires-mediated changes in renal gene expression to identify biological pathways associated with kidney damage. Results Leptospiral genes were detected in renal transcriptomes of mice infected with Leptospira interrogans at day 28 post-infection, suggesting colonization of leptospires within the kidney with propensity of chronicity. Comparative differential gene expression and pathway analysis were investigated in renal transcriptomes of mice infected with pathogens and non-pathogens. Pathways analysis showed that Toll-like receptor signaling, complements activation, T-helper 1 type immune response and T cell mediated immunity/chemotaxis/proliferation were strongly associated with progressive tubulointerstitial damage caused by pathogenic leptospiral infection. Additionally, 26 genes related with complement system, immune function and cell-cell interactions were found to be significantly up-regulated in the L. interrogans-infected renal transcriptome. Conclusions Our results provided comprehensive knowledge regarding the host transcriptional response to leptospiral infection in murine kidneys, particularly the involvement of cell-to-cell interaction in the immune response. It would provide valuable resources to explore functional studies of chronic renal damage caused by leptospiral infection.
原文American English
頁(從 - 到)1411-1423
頁數13
期刊Journal of Infectious Diseases
218
發行號9
DOIs
出版狀態Published - 1 十一月 2018

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