Multiphoton imaging of dye penetration dynamics in tissue sections

Po Hang Tseng, Shu Jen Chiang, Shean Jen Chen, Chen Yuan Dong*

*Corresponding author for this work

研究成果: Conference contribution同行評審

摘要

Rapid and in-depth labeling biological tissue samples is invaluable for studying tissue organization in three-dimensions. The current strategy in finding optimal labeling parameters for dye molecules through tissues often requires trial and error testing. Difficulty in establishing a standard approach is due to lack of information on the diffusion parameters of dye through tissues. In this study we investigate the temporal progression of dye penetration in different tissue types. By the combination of multiphoton imaging of labeled tissues and application of the theoretical model of diffusion with well-defined boundary conditions, we aim to arrive at systematic parameters for optimizing dye penetration. Determination of the effective diffusion coefficient of the dyes used will then be useful for further application.

原文English
主出版物標題Multiphoton Microscopy in the Biomedical Sciences XX
編輯Ammasi Periasamy, Peter T. C. So, Karsten Konig, Karsten Konig
發行者SPIE
ISBN(電子)9781510632516
DOIs
出版狀態Published - 1 一月 2020
事件Multiphoton Microscopy in the Biomedical Sciences XX 2020 - San Francisco, United States
持續時間: 2 二月 20204 二月 2020

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
11244
ISSN(列印)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XX 2020
國家United States
城市San Francisco
期間2/02/204/02/20

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