Investigating the secondary structures for long oligonucleotides using attenuated-total-reflection nanoplasmon-enhanced Raman scattering

K. C. Chiu*, L. Y. Yu, C. Y. Lin, S. J. Chen

*Corresponding author for this work

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

This study utilizes a nanoplasmon-enhanced Raman scattering based on the attenuated-total-reflection (ATR) method to investigate the secondary structures of long oligonucleotides and their influence on the DNA hybridization. It is found that the ring-breathing modes of adenine, thymine, guanine, and cytosine in Raman fingerprint associated with three 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35°C to 45°C reduces secondary structure effects. The kinetics of biomolecular interaction analysis can be performed by using surface plasmons resonance biosensor, but the structural information of the oligonucleotides can not observed directly. The ATR-Raman spectrum can provide the structural information of the oligonucleotide monolayer on the sensing surface with the help of a silver patterned nanostructure film based on the finite-difference time-domain simulation and the e-beam lithography fabrication adapted as an ATR-Raman active substrate.

原文English
主出版物標題Plasmonics
主出版物子標題Nanoimaging, Nanofabrication, and Their Applications III
DOIs
出版狀態Published - 1 十二月 2007
事件Plasmonics: Nanoimaging, Nanofabrication, and Their Applications III - San Diego, CA, United States
持續時間: 28 八月 200730 八月 2007

出版系列

名字Proceedings of SPIE - The International Society for Optical Engineering
6642
ISSN(列印)0277-786X

Conference

ConferencePlasmonics: Nanoimaging, Nanofabrication, and Their Applications III
國家United States
城市San Diego, CA
期間28/08/0730/08/07

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