Influence of the condenser on sample tracking via forward scattering pattern detection

Che Liang Tsai*, Bo Jui Chang, Kuang Lung Huang, Long Hsu

*Corresponding author for this work

研究成果: Conference article同行評審

摘要

Sample tracking with a high spatial sensitivity is highly desired in force measurement with optical tweezers. However, the trick that sample tracking via forward scattering pattern detection would provide a higher sensitivity than that via regular image detection has never been investigated. In this paper, we systematically study the influences of the position and the numerical aperture of the condenser on sample tracking via forward scattering pattern detection. In our experiment, a 60X condenser is used to form the forward scattering pattern of a sample bead upon a CCD camera. As the bead is transversely shifted at a step size of 30nm by a PZT XYZ stage, we measure the magnitude of the corresponding shift of the forward scattering pattern when the 60X condenser of different angular apertures is placed at various locations along the optical axis. Our result shows that the most sensitive forward scattering pattern occurs when the condenser collimates the forward scattering light from the sample bead. We also find that the larger the numerical aperture is, the higher the sensitivity of forward scattering pattern detection will be.

原文English
文章編號54
頁(從 - 到)393-401
頁數9
期刊Proceedings of SPIE - The International Society for Optical Engineering
5514
DOIs
出版狀態Published - 1 十二月 2004
事件Optical Trapping and Optical Micromanipulation - Denver, CO, United States
持續時間: 2 八月 20046 八月 2004

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