Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy

Lun De Liao, Meng Lin Li, Hsin Yi Lai, Yen Yu I. Shih, Yu Chun Lo, Siny Tsang, Paul C.-P. Chao, Chin Teng Lin, Fu Shan Jaw, You Yin Chen*

*Corresponding author for this work

研究成果: Article同行評審

87 引文 斯高帕斯(Scopus)

摘要

The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO2). Changes in CBV were measured by images acquired at a wavelength of 570nm (Λ570), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO2 changes were sensed by pixel-wise normalization of images acquired at Λ560 or Λ600 to those at Λ570. We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO2 and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models.

原文English
頁(從 - 到)562-570
頁數9
期刊NeuroImage
52
發行號2
DOIs
出版狀態Published - 1 八月 2010

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