Human cytomegalovirus preferentially infects the neoplastic epithelium of colorectal cancer: A quantitative and histological analysis

Hsin Pai Chen, Jeng Kai Jiang, Cheng Yu Chen, Teh Ying Chou, Yen Chung Chen, Ya Ting Chang, Shiou Fu Lin, Chia Hao Chan, Chih Yung Yang, Chi Hung Lin, Jen Kou Lin, Wen Long Cho*, Yu Jiun Chan

*Corresponding author for this work

研究成果: Article同行評審

32 引文 斯高帕斯(Scopus)

摘要

Background: It has long been suggested that human cytomegalovirus (HCMV) might be involved in human oncogenesis. However, whether HCMV was associated with colorectal cancer (CRC) was still controversial. Objective: To clarify whether HCMV specifically infects the tumorous tissue of CRC. Study design: Paired tumor and adjacent non-neoplastic CRC specimens were collected from 163 patients. HCMV DNA was detected and quantified through PCR and quantitative real-time PCR. Virus location was determined by in situ hybridization (ISH) of formalin-fixed paraffin-embedded tissue sections with an HCMV-specific probe. Results: By PCR, HCMV DNA was detected in 42.3% (69/163) of the tumor specimens, while only 5.6%(14/163) samples of adjacent non-neoplastic tissue were positive for HCMV (p< 0.0001). Quantitative real-time PCR in 54 sample pairs revealed significantly higher viral copies in the tumor specimens than the adjacent non-neoplastic tissue specimens (p< 0.001). By ISH, the nucleic acids of HCMV were detected in the cytoplasm of neoplastic epithelium. No hybridization was detected in the inflammatory infiltrates, submucosa, or other stromal tissues. Conclusions: HCMV preferentially infects the tumor epithelium of CRC. How the virus subsists in and interacts with the microenvironment of tumor epithelium of CRC should be studied.

原文English
頁(從 - 到)240-244
頁數5
期刊Journal of Clinical Virology
54
發行號3
DOIs
出版狀態Published - 七月 2012

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