High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors

Ming Yu Lin, Yu Wei Chang, Yu Ting Tai, Yuh-Shyong Yang, Yang Tung Huang

研究成果: Conference contribution同行評審

1 引文 斯高帕斯(Scopus)

摘要

Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.

原文English
主出版物標題Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008
頁面86-89
頁數4
DOIs
出版狀態Published - 1 十二月 2008
事件3rd International Conference on Sensing Technology, ICST 2008 - Tainan, Taiwan
持續時間: 30 十一月 20083 十二月 2008

出版系列

名字Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008

Conference

Conference3rd International Conference on Sensing Technology, ICST 2008
國家Taiwan
城市Tainan
期間30/11/083/12/08

指紋 深入研究「High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors」主題。共同形成了獨特的指紋。

引用此