Functional magnetic nanoparticles–assisted electrochemical biosensor for eosinophil cationic protein in cell culture

Cheng Yu Lee, Li Ping Wu, Tzu Ting Chou, You-Zung Hsieh*

*Corresponding author for this work

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

A low-cost electrochemical biosensor, assisted by a new type of functional magnetic nanoparticles (NPs), has been developed to detect eosinophil cationic protein (ECP), a known biomarker of asthma, in cell culture. The heparin-modified magnetic NPs were mixed with a sample solution containing ECP. After ECP had been captured by the NPs, a magnetic field was applied behind a graphite-based screen-printed electrode. The functional magnetic NPs were attracted to the electrode, raising the ECP concentration near its surface. Because of the use of the functional magnetic NPs, the difference in the signal was amplified when applying larger sample volumes for detection, thereby enhancing the sensitivity of the biosensor. This approach provided a linear range for the analysis of the logarithm of the ECP concentration from 1 to 1000 nM, with a coefficient of determination 0.992; the limit of detection was 0.30 nM. The fabricated biosensor displayed good recovery in a cell culture medium incubated with the Beas–2 B cell line. The ability to detect the concentration of ECP in a cell culture at any time point should be useful for explaining contradictory findings regarding the relationship between the initial ECP concentration and the cell line.

原文English
頁(從 - 到)672-677
頁數6
期刊Sensors and Actuators, B: Chemical
257
DOIs
出版狀態Published - 1 三月 2018

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