Effective extraction and purification of β-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioning

I. Horng Pan, Hsin Jan Yao, Yaw-Kuen Li*

*Corresponding author for this work

研究成果: Article同行評審

33 引文 斯高帕斯(Scopus)

摘要

Effective extraction of protein from bulk medium is an important technique in bioresearch. In the present study, we describe an extracellular β-xylosidase from the fermentation supernatant of Trichoderma koningii G-39 that was successfully extracted and purified simultaneously in a single step by using an aqueous two-phase partitioning method. This two-phase system was prepared by dissolving suitable amount of poly(ethylene glycol) (PEG) and sodium dihydrogenphosphate (NaH2PO4) in aqueous solution. β-Xylosidase was recovered with high yield and high concentration in the bottom salt-rich phase when 25% (w/v) PEG 1500 and 20-25% (w/v) NaH2PO4 were applied. Based on a 1-liter scale extraction, the purity of the enzyme was enhanced at least 33-fold. The total activity increased 422% in comparison with that in the untreated filtrate. The effectiveness and simplicity may make this technique potentially useful in various applications. The transxylosylation activity of the enzyme purified by this technique was also investigated.

原文English
頁(從 - 到)196-201
頁數6
期刊Enzyme and Microbial Technology
28
發行號2-3
DOIs
出版狀態Published - 1 二月 2001

指紋 深入研究「Effective extraction and purification of β-xylosidase from Trichoderma koningii fermentation culture by aqueous two-phase partitioning」主題。共同形成了獨特的指紋。

引用此