Dynamic and programmable cell-sorting by using microfluidics and holographic optical tweezers

Ho Chien Lin*, Long Hsu

*Corresponding author for this work

研究成果: Conference article同行評審

1 引文 斯高帕斯(Scopus)

摘要

Cell sorting is important in quantitative analysis for cellular and molecular biology study. For this purpose, we develop an opto-electro-mechanical sorting system which integrates a holographic optical tweezers (HOT) system to a microfluidics device. This system is designed to manipulate and sort various micro objects of different sizes by an optical pattern which is dynamic and programmable. In this work, we fabricate a microchannel with a main channel and two branches with polydimethylsiloxane (PDMS) which is a biocompatible elastomer material by utilizing semiconductor micro-fabrication techniques. By using a HOT system, we also generate two linear optical traps for the manipulation of micro particles. As a result, we have demonstrated that the larger particles are guided to the second branch by the two linear optical traps while the smaller particles remain unaffected and flow into the first branch of the microchannel.

原文English
文章編號59301V
頁(從 - 到)1-8
頁數8
期刊Proceedings of SPIE - The International Society for Optical Engineering
5930
DOIs
出版狀態Published - 1 十二月 2005
事件Optical Trapping and Optical Micromanipulation II - San Diego, CA, United States
持續時間: 31 七月 20054 八月 2005

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