DNA unwinding ability of Xenopus transcription factor A

Jay S. Hanas*, Daniel F. Bogenhagen, Cheng-Wen Wu

*Corresponding author for this work

研究成果: Article

15 引文 斯高帕斯(Scopus)

摘要

The duplex DNA unwinding ability of Xenopus transcription factor A was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Factor A binding to plasmids does result in a small degree of unwinding as evidenced by a shift in topoisomer distributions upon relaxation. Slightly more unwinding by factor A was observed on a specific plasmid, containing four tandemly repeated 5S RNA genes, when compared to a non-specific plasmid of nearly identical size containing no 5S specific sequences. The amount of unwinding observed on the specific plasmid, about one base-pair per gene, suggests that unwinding of the DNA duplex is not an important aspect of binding of factor A to the 5S RNA gene.

原文English
頁(從 - 到)1265-1276
頁數12
期刊Nucleic acids research
12
發行號2
DOIs
出版狀態Published - 25 一月 1984

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