Direct observation of thymine dimer repair in DNA by photolyase

Ya-Ting Kao, Chaitanya Saxena, Lijuan Wang, Aziz Sancar*, Dongping Zhong

*Corresponding author for this work

研究成果: Article同行評審

206 引文 斯高帕斯(Scopus)

摘要

Photolyase uses light energy to split UV-induced cyclobutane dimers in damaged DNA, but its molecular mechanism has never been directly revealed. Here, we report the direct mapping of catalytic processes through femtosecond synchronization of the enzymatic dynamics with the repair function. We observed direct electron transfer from the excited flavin cofactor to the dimer in 170 ps and back electron transfer from the repaired thymines in 560 ps. Both reactions are strongly modulated by active-site solvation to achieve maximum repair efficiency. These results show that the photocycle of DNA repair by photolyase is through a radical mechanism and completed on subnanosecond time scale at the dynamic active site, with no net change in the redox state of the flavin cofactor.

原文English
頁(從 - 到)16128-16132
頁數5
期刊Proceedings of the National Academy of Sciences of the United States of America
102
發行號45
DOIs
出版狀態Published - 8 十一月 2005

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