Computational prediction of new intein split sites

Yi Zong Lee, Wei-Cheng Lo*, Shih Che Sue

*Corresponding author for this work

研究成果: Chapter同行評審

2 引文 斯高帕斯(Scopus)

摘要

Split inteins have emerged as a powerful tool in protein engineering. We describe a reliable in silico method to predict viable split sites for the design of new split inteins. A computational circular permutation (CP) prediction method facilitates the search for internal permissive sites to create artificial circular permutants. In this procedure, the original amino-and carboxyl-termini are connected and new termini are created. The identified new terminal sites are promising candidates for the generation of new split sites with the backbone opening being tolerated by the structural scaffold. Here we show how to integrate the online usage of the CP predictor, CPred, in the search of new split intein sites.

原文English
主出版物標題Methods in Molecular Biology
發行者Humana Press Inc.
頁面259-268
頁數10
DOIs
出版狀態Published - 1 一月 2017

出版系列

名字Methods in Molecular Biology
1495
ISSN(列印)1064-3745

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