Canine transmissible venereal tumor cell depletion of B lymphocytes: Molecule(s) specifically toxic for B cells

Kuang-Wen Liao, Shao Wen Hung, Ya Wen Hsiao, Michael Bennett, Rea Min Chu*

*Corresponding author for this work

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)

摘要

Canine transmissible venereal tumor (CTVT) is an excellent model for investigating the interaction between host immunity and tumor growth. Although CTVT is an allograft, initially the host immune system is unable to destroy the tumor cells, and the tumor grows progressively for about 4-6 months (P phase). After a short stable phase, the tumor undergoes regression (R phase). In this study, CTVT inoculation significantly reduced the proportion of B lymphocytes among all peripheral blood lymphocytes (PBL), but the proportion of B lymphocytes returned to normal after complete removal of CTVT. Following CTVT inoculation, immunoglobulin concentrations decreased gradually, coincident with B lymphocyte decline. Furthermore, CTVT secreted a soluble, heat- and protease K-sensitive cytotoxic molecule(s) that destroyed peripheral blood B lymphocytes (PBBL) but spared other types of immune cells regardless of whether mitogens, such as IL-2 or Con A, were present. The decrease in the proportion and viability of PBBL was caused by a cytotoxic molecule(s) that induced apoptosis. The molecular weight of the CTVT-derived cytotoxic molecule(s) was 30-100 kDa. Human, domestic cat, horse and mouse B cells were also sensitive to the substance.

原文English
頁(從 - 到)149-162
頁數14
期刊Veterinary Immunology and Immunopathology
92
發行號3-4
DOIs
出版狀態Published - 12 五月 2003

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