An investigation into the influence of secondary structures for DNA hybridization using surface plasmon resonance and surface-enhanced Raman scattering

J. N. Yih*, K. C. Chiu, F. C. Chien, W. Y. Chen, Shean-Jen Chen

*Corresponding author for this work

研究成果: Conference contribution

3 引文 斯高帕斯(Scopus)

摘要

This study utilizes a surface plasmon resonance (SPR) biosensing to investigate the influence of secondary structures on the DNA hybridization and a surface-enhanced Raman scattering (SERS) spectrum to yield analytical data regarding the structure of the oligonucleotides. It is found that the SPR angular shifts associated with the three pairs of 60mer oligonucleotides with prominent secondary structures are lower than those observed for the two pairs of oligonucleotides with no obvious secondary structures. It is also determined that increasing the DNA hybridization temperature from 35°C to 45°C reduces secondary structure effects. On the hybridization with mixture target oligonucleotides, the SPR results demonstrate that secondary structures interfere significantly. Although the kinetics of biomolecular interaction analysis is performed by using SPR sensor, the structural information of the oligonucleotides can not observed directly. The SERS spectrum provides the structural information of the oligonucleotides with silver colloidal nanoparticles adapted as a Raman active substrate. Also, the detection limit of the DNA Raman signal has been successfully improved to reach sub-micro molarity of DNA concentration.

原文English
主出版物標題Plasmonics in Biology and Medicine III
DOIs
出版狀態Published - 26 六月 2006
事件Plasmonics in Biology and Medicine III - San Jose, CA, United States
持續時間: 23 一月 200624 一月 2006

出版系列

名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6099
ISSN(列印)1605-7422

Conference

ConferencePlasmonics in Biology and Medicine III
國家United States
城市San Jose, CA
期間23/01/0624/01/06

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    Yih, J. N., Chiu, K. C., Chien, F. C., Chen, W. Y., & Chen, S-J. (2006). An investigation into the influence of secondary structures for DNA hybridization using surface plasmon resonance and surface-enhanced Raman scattering. 於 Plasmonics in Biology and Medicine III [609906] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; 卷 6099). https://doi.org/10.1117/12.647449