Actin waves transport RanGTP to the neurite tip to regulate non-centrosomal microtubules in neurons

Yung An Huang, Chih Hsuan Hsu, Ho Chieh Chiu, Pei Yu Hsi, Chris T. Ho, Wei Lun Lo, Eric Hwang

研究成果: Article同行評審

1 引文 斯高帕斯(Scopus)

摘要

Microtubules (MTs) are the most abundant cytoskeleton in neurons, and control multiple facets of their development. While the MT-organizing center (MTOC) in mitotic cells is typically located at the centrosome, the MTOC in neurons switches to non-centrosomal sites. A handful of cellular components have been shown to promote non-centrosomal MT (ncMT) formation in neurons, yet the regulation mechanism remains unknown. Here, we demonstrate that the small GTPase Ran is a key regulator of ncMTs in neurons. Using an optogenetic tool that enables light-induced local production of RanGTP, we demonstrate that RanGTP promotes ncMT plus-end growth along the neurite. Additionally, we discovered that actin waves drive the anterograde transport of RanGTP. Pharmacological disruption of actin waves abolishes the enrichment of RanGTP and reduces growing ncMT plus-ends at the neurite tip. These observations identify a novel regulation mechanism for ncMTs and pinpoint an indirect connection between the actin and MT cytoskeletons in neurons.

原文English
期刊Journal of cell science
133
發行號9
DOIs
出版狀態Published - 14 五月 2020

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