A ZnO nanogenerator of thickness 12.5 μm has been fabricated in this study. The ZnO nanogenerator is composed of ZnO nanowires embedded in PDMS layer, which is highly flexible and lightweight can be vibrate by wind force at the air flow rate of 0.5 ms-1. Thus, the ZnO nanogenerator can be a wind speed sensor for human respiration detector. The open-circuit voltage is about 41mV for a normal human respiration at rest with the tidal volume of 500 mL and the air flow rate of 2.0 ms-1. The open-circuit voltage and closed-circuit current-density reached the highest value at the air flow rate of 5.0 ms-1, 61.2 mV and 7.12 nAcm-2, respectively. With increasing tidal volume and an air flow rate of 2.0 ms-1, the electrical performance of the ZnO nanogenerator was 81.3 mV and 11.4 nAcm -2, respectively.