YjcC, a c-di-GMP Phosphodiesterase Protein, Regulates the Oxidative Stress Response and Virulence of Klebsiella pneumoniae CG43

Ching Jou Huang, Zhe Chong Wang, Hsi Yuan Huang, Hsien Da Huang, Hwei-Ling Peng*

*Corresponding author for this work

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

This study shows that the expression of yjcC, an in vivo expression (IVE) gene, and the stress response regulatory genes soxR, soxS, and rpoS are paraquat inducible in Klebsiella pneumoniae CG43. The deletion of rpoS or soxRS decreased yjcC expression, implying an RpoS- or SoxRS-dependent control. After paraquat or H2O2 treatment, the deletion of yjcC reduced bacterial survival. These effects could be complemented by introducing the ΔyjcC mutant with the YjcC-expression plasmid pJR1. The recombinant protein containing only the YjcC-EAL domain exhibited phosphodiesterase (PDE) activity; overexpression of yjcC has lower levels of cyclic di-GMP. The yjcC deletion mutant also exhibited increased reactive oxygen species (ROS) formation, oxidation damage, and oxidative stress scavenging activity. In addition, the yjcC deletion reduced capsular polysaccharide production in the bacteria, but increased the LD50 in mice, biofilm formation, and type 3 fimbriae major pilin MrkA production. Finally, a comparative transcriptome analysis showed 34 upregulated and 29 downregulated genes with the increased production of YjcC. The activated gene products include glutaredoxin I, thioredoxin, heat shock proteins, chaperone, and MrkHI, and proteins for energy metabolism (transporters, cell surface structure, and transcriptional regulation). In conclusion, the results of this study suggest that YjcC positively regulates the oxidative stress response and mouse virulence but negatively affects the biofilm formation and type 3 fimbriae expression by altering the c-di-GMP levels after receiving oxidative stress signaling inputs.

Original languageEnglish
Article numbere66740
JournalPLoS ONE
Volume8
Issue number7
DOIs
StatePublished - 23 Jul 2013

Keywords

  • ESCHERICHIA-COLI K-12; GENE-EXPRESSION; SUPEROXIDE-DISMUTASE; DIGUANYLATE CYCLASE; SIGNAL-TRANSDUCTION; TWITCHING MOTILITY; BIOFILM FORMATION; LIVER-ABSCESS; FUMARASE FUMA; EAL DOMAIN

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