Xenopus transcription factor A requires zinc for binding to the 5 S RNA gene.

J. S. Hanas*, D. J. Hazuda, D. F. Bogenhagen, F. Y. Wu, Cheng-Wen Wu Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

234 Scopus citations

Abstract

Transcription factor A from immature Xenopus oocytes is found associated with 5 S RNA in a 7 S nucleoprotein complex. Atomic absorption analysis of EDTA-dialyzed 7 S particles reveals 2 mol of zinc/mol of particle. Factor A obtained from EDTA-dialyzed particles binds specifically to the 5 S RNA gene as determined by DNase I footprinting. Factor A alone, obtained by RNase digestion of the 7 S particle, contains zinc when dialyzed in the absence of EDTA. However, the zinc bound to free factor A is removed by dialysis against a buffer containing EDTA. The apoprotein does not bind to the 5 S RNA gene. Inhibition of footprinting is also effected by addition of EDTA to factor A without prolonged dialysis. Under these conditions, specific DNA binding ability is restored following addition of zinc. 1,10-Phenanthroline also inhibits binding of factor A to the intragenic control region of the 5 S RNA gene. In addition, this reagent specifically inhibits factor A-dependent synthesis of 5 S RNA but not factor A-independent tRNA synthesis in a HeLa cell in vitro transcription system.

Original languageEnglish
Pages (from-to)14120-14125
Number of pages6
JournalJournal of Biological Chemistry
Volume258
Issue number23
StatePublished - 10 Dec 1983

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