Volume-oriented sample preparation for reactant minimization on flow-based microfluidic biochips with multi-segment mixers

Chi Mei Huang, Chia Hung Liu, Juinn-Dar Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Scopus citations

Abstract

Sample preparation is one of essential processes in most biochemical reactions. In this process, raw reactants are diluted to achieve given target concentrations. So far, most of existing sample preparation techniques only consider mixing of two source solutions under the (1:1) mixing model. In this paper, we propose the first sample preparation algorithm VOSPA that not only blends several (≥ 2) solutions in a dilution operation but also allows various mixing models on flow-based microfluidic biochips with multi-segment mixers. VOSPA is a volume-oriented sample preparation algorithm that enables segment-based intermediate solution reuse for better reactant minimization. Experimental results show that VOSPA can lower the reactant consumption and operation count by 72% and 59% as compared to the baseline bit-scanning method if an 8-segment mixer is used. Moreover, VOSPA outperforms an optimal algorithm, which merely allows the use of (1:1) mixing model; the reactant usage and operation count can be further reduced by 37% and 76%.

Original languageEnglish
Title of host publicationProceedings of the 2015 Design, Automation and Test in Europe Conference and Exhibition, DATE 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1114-1119
Number of pages6
ISBN (Electronic)9783981537048
StatePublished - 22 Apr 2015
Event2015 Design, Automation and Test in Europe Conference and Exhibition, DATE 2015 - Grenoble, France
Duration: 9 Mar 201513 Mar 2015

Publication series

NameProceedings -Design, Automation and Test in Europe, DATE
Volume2015-April
ISSN (Print)1530-1591

Conference

Conference2015 Design, Automation and Test in Europe Conference and Exhibition, DATE 2015
CountryFrance
CityGrenoble
Period9/03/1513/03/15

Keywords

  • Biochip
  • dilution
  • flow-based microfluidic biochip
  • mixing model
  • reactant minimization
  • sample preparation

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    Huang, C. M., Liu, C. H., & Huang, J-D. (2015). Volume-oriented sample preparation for reactant minimization on flow-based microfluidic biochips with multi-segment mixers. In Proceedings of the 2015 Design, Automation and Test in Europe Conference and Exhibition, DATE 2015 (pp. 1114-1119). [7092555] (Proceedings -Design, Automation and Test in Europe, DATE; Vol. 2015-April). Institute of Electrical and Electronics Engineers Inc..