Viability evaluation of culture cells patterned by femtosecond laser-induced impulsive force

Noriko Takizawa*, Kazunori Okano, Takayuki Uwada, Yoichiroh Hosokawa, Hiroshi Masuhara

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations

Abstract

PC12 cells, which are derived from a rat pheochromocytoma, were independently patterned utilizing an impulsive force resulting in impulsive Shockwave and cavitation bubble generation by focused femtosecond laser irradiation. Since the PC 12 cells respond reversibly to nerve growth factor by induction of the neuronal phenotype, we can assess an influence that the impulsive force gives to the bioactivity in term of the cell differentiation. The patterned cells were accumulated on an intact dish and cultured for 3 days. The behavior of appearance and cell differentiation was observed by multipoint time-lapse system. On bases of these results, it was proved that the biological activity of the cell is unaffected by the femtosecond laser patterning.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XIX
DOIs
StatePublished - 21 Apr 2008
EventOptical Interactions with Tissue and Cells XIX - San Jose, CA, United States
Duration: 21 Jan 200823 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6854
ISSN (Print)1605-7422

Conference

ConferenceOptical Interactions with Tissue and Cells XIX
CountryUnited States
CitySan Jose, CA
Period21/01/0823/01/08

Keywords

  • Cavitation bubble
  • Cell patterning
  • Differentiation
  • Femtosecond laser
  • Multi-point time-lapse
  • Multiphoton excitation
  • Nerve growth factor
  • PC 12
  • Shockwave

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    Takizawa, N., Okano, K., Uwada, T., Hosokawa, Y., & Masuhara, H. (2008). Viability evaluation of culture cells patterned by femtosecond laser-induced impulsive force. In Optical Interactions with Tissue and Cells XIX [685411] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6854). https://doi.org/10.1117/12.762584