Using silicon nanowire devices to detect adenosine triphosphate liberated from electrically stimulated HeLa cells

C. C. Chen, Y. Z. Chen, Y. J. Huang, Jeng-Tzong Sheu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

In this study, we used a biosensor chip featuring Abl tyrosine kinase-modified silicon nanowire field-effect transistors (SiNW-FETs) to detect adenosine triphosphate (ATP) liberated from HeLa cells that had been electrically stimulated. Cells that are cultured in high-ionic-strength media or buffer environments usually undermine the sensitivity and selectively of SiNW-FET-based sensors. Therefore, we first examined the performance of the biosensor chip incorporating the SiNW-FETs in both low- and high-ionic-strength buffer solutions. Next, we stimulated, using a sinusoidal wave (1.0. V, 50. Hz, 10. min), HeLa cells that had been cultured on a cell-culture chip featuring interdigitated electrodes. The extracellular ATP concentration increased by ca. 18.4-fold after electrical stimulation. Finally, we detected the presence of extracellular ATP after removing a small amount of buffer solution from the cell-cultured chip and introducing it into the biosensor chip.

Original languageEnglish
Pages (from-to)2323-2328
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
StatePublished - 15 Jan 2011

Keywords

  • Adenosine triphosphate
  • HeLa cell
  • Ionic strength
  • Silicon nanowire field-effect transistor

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