Using lactosylated cysteine functionalized gold nanoparticles as colorimetric sensing probes for rapid detection of the ricin B chain

Karthikeyan Kandasamy, Karuppuchamy Selvaprakash, Yu Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

A new colorimetric method that can be used to rapidly detect toxic ricin is demonstrated. Lactosylated cysteine-functionalized gold nanoparticles (Au@LACY NPs) were prepared by a one-pot reaction and employed as optical probes for determination of ricin B chain. It is found that the Au@LACY NPs undergo aggregation in the presence of ricin B chain. This leads to surface plasmon coupling effects of the particles and a color change from red to blue, with absorption maxima at 519 and 670 nm, respectively. The feasibility of using the current approach for quantitative analysis of ricin B chain is also demonstrated. The calibration plot is generated by plotting the ratio of the absorbance at the wavelength of 634 to 518 nm versus the concentration of the ricin B chain. The spectrophotometric method has a ~29 pM (~ 0.91 ng·mL−1) detection limit, and the sample with the concentration of ~ 400 pM (~ 13 ng·mL−1) can be detected visually. [Figure not available: see fulltext.].

Original languageEnglish
Article number847
JournalMicrochimica Acta
Volume186
Issue number12
DOIs
StatePublished - 27 Nov 2019

Keywords

  • Bioterrorism agent
  • Castor plant
  • Lactose
  • Localized surface plasmon
  • Surface plasmon coupling effects

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