Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

Chian-Shiu Chien, Chien-Ying Wang, Yi-Ping Yang, Shih-Jie Chou, Yu-Ling Ko, Fu-Ting Tsai, Wen-Chung Yu, Chia-Ching Chang, Jong-Yuh Cherng, Meng-Yin Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background:

Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.

Methods:

The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.

Results:

Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.

Conclusion:

We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalJournal of the Chinese Medical Association
Volume83
Issue number4
DOIs
StatePublished - Apr 2020

Keywords

  • Induced pluripotent stem cells
  • Non-viral gene delivery
  • Polyurethane short-branch polyethylenimine
  • TRANSFECTION
  • PROLIFERATION

Cite this