Using cationic polyurethane-short branch PEI as microRNA-driven nano-delivery system for stem cell differentiation

Chian-Shiu Chien, Chien-Ying Wang, Yi-Ping Yang, Shih-Jie Chou, Yu-Ling Ko, Fu-Ting Tsai, Wen-Chung Yu, Chia-Ching Chang, Jong-Yuh Cherng, Meng-Yin Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations



Non-viral gene delivery, such as using biodegradable polyurethane short-branch polyethylenimine (PU-PEI), has been considered a potentially safer gene delivery system in comparison to conventional virus systems.


The polycationization of DNA complexes protects DNA from nuclease degradation, and these DNA complexes are nanoscale in size to enter the cell through endocytosis.


Due to the net positive surface charge of the cell, these polyplexes efficiently bind to the cell through electrostatic interactions with negatively charged membrane components. Cationic PU-PEI has been shown to be non-cytotoxic and has a high transfection efficiency, making it a practical gene delivery material in diseases.


We developed a PU-PEI nanomedicine-based platform to efficiently deliver microRNA in promoting differentiation capacity of stem cells, especially on induced pluripotent stem cells.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalJournal of the Chinese Medical Association
Issue number4
StatePublished - Apr 2020


  • Induced pluripotent stem cells
  • Non-viral gene delivery
  • Polyurethane short-branch polyethylenimine

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