Using cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography to determine selective serotonin reuptake inhibitors

Hsiu L. Su, You-Zung Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We have employed a rapid and highly efficient on-line preconcentration method, cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography (CSEI-sweeping-MEKC), for the analysis of selective serotonin reuptake inhibitors (SSRIs) of antidepressant drugs. We monitored the effects of several of the CSEI-sweeping-MEKC parameters - including the pH, the concentrations of high-conductivity buffer (HCB), sodium dodecyl sulfate (SDS), and organic modifier, the injection length of the HCB, and the injection time of the sample - to optimize the separation process. The optimal background electrolyte was 50 mM citric acid/disodium hydrogenphosphate buffer (pH 2.2) containing 100 mM SDS and 22% isopropyl alcohol. The sensitivity enhancements of the SSRIs sertraline, fluoxetine, paroxetine, fluvoxamine, and citalopram ranged from 5.7 × 104 to 1.2 × 105; the coefficients of determination exceeded 0.9938 and the relative standard deviations of the peak heights were less than 3.2%; the detection limits ranged from 0.056 to 0.22 ng/mL. We employed the optimal conditions to analyze these five SSRIs in a plasma sample prepared using solid-phase extraction (SPE) to minimize the influence of the matrix. Although the limits of detection of the SSRIs in human plasma were higher than those in pure water, this present technique is more sensitive than other, more-conventional methods. The recovery of the SPE extraction efficiency was satisfactory (up to 89%). Our findings suggest that, under the optimal conditions, the CSEI-sweeping-MEKC method can be used successfully to determine these five SSRIs in human plasma.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalJournal of Chromatography A
Volume1209
Issue number1-2
DOIs
StatePublished - 31 Oct 2008

Keywords

  • Antidepressant drug
  • CSEI-sweeping-MEKC
  • MEKC
  • On-line preconcentration
  • Selective serotonin reuptake inhibitor

Fingerprint Dive into the research topics of 'Using cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography to determine selective serotonin reuptake inhibitors'. Together they form a unique fingerprint.

Cite this