Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma

Huey Huey Chua, Te Huei Yeh, Ying Piao Wang, Yu Tzu Huang, Tzung Shiahn Sheen, You Chang Lo, Ya-Ching Chou, Ching Hwa Tsai*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Background. Nasopharyngeal carcinoma (NPC) is associated with Epstein-Barr virus (EBV) and has high metastatic potential. Discoidin domain receptors (DDR1, DDR2) are receptor-type tyrosine kinases activated by collagen. Their ability to induce expression of matrix metalloproteinase is related with tumor invasion. Therefore, we aim to investigate DDRs gene expression and its regulation in NPC. Methods and Results. By use of real-time quantitative polymerase chain reaction (Q-PCR), DDR2 gene expression but not DDR1 was significantly higher in primary and metastatic NPC. DDR2 was predominantly distributed in NPC tumor cells rather than in infiltrating lymphocytes. EBV Z-transactivator (Zta) transfection may distinctly elevate DDR2 level. Furthermore, data from reporter assay indicate that Zta could transactivate DDR2 promoter activity, suggesting the possible upregulation mechanism. Conclusion. DDR2 was differentially upregulated in NPC and modulated by EBV Zta protein. DDR2 may play a role in NPC invasion and serve as a diagnostic and therapeutic target.

Original languageEnglish
Pages (from-to)427-436
Number of pages10
JournalHead and Neck
Volume30
Issue number4
DOIs
StatePublished - Apr 2008

Keywords

  • Discoidin domain receptor 2
  • Discoidin domain receptor gene family
  • Epstein-Barr virus
  • Nasopharyngeal carcinoma
  • Z-transactivator

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    Chua, H. H., Yeh, T. H., Wang, Y. P., Huang, Y. T., Sheen, T. S., Lo, Y. C., Chou, Y-C., & Tsai, C. H. (2008). Upregulation of discoidin domain receptor 2 in nasopharyngeal carcinoma. Head and Neck, 30(4), 427-436. https://doi.org/10.1002/hed.20724