Ultrasonication-assisted spray ionization mass spectrometry for the analysis of biomolecules in solution

Tsung Yi Chen, Jia Yi Lin, Jen Yi Chen, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


In this paper, we describe a novel technique-ultrasonication-assisted spray ionization (UASI)-for the generation of singly charged and multiply charged gas-phase ions of biomolecules (e.g., amino acids, peptides, and proteins) from solution; this method employs a low-frequency ultrasonicator (ca. 40 kHz) in place of the high electric field required for electrospray ionization. When a capillary inlet is immersed into a sample solution within a vial subjected to ultrasonication, the solution is continually directed to the capillary outlet as a result of ultrasonication-assisted capillary action; an ultrasonic spray of the sample solution is emitted at the outlet of the tapered capillary, leading to the ready generation of gas-phase ions. Using an ion trap mass spectrometer, we found that singly charged amino acid and multiply charged peptides/proteins ions were generated through this single-step operation, which is both straightforward and extremely simple to perform. The setup is uncomplicated: only a low-frequency ultrasonicator and a tapered capillary are required to perform UASI. The mass spectra of the multiply charged peptides and proteins obtained from sample solutions subjected to UASI resemble those observed in ESI mass spectra.

Original languageEnglish
Pages (from-to)1547-1553
Number of pages7
JournalJournal of the American Society for Mass Spectrometry
Issue number9
StatePublished - 1 Sep 2010

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