Ultrafast relaxation dynamics of nitric oxide synthase studied by visible broadband transient absorption spectroscopy

Chih Chang Hung, Atsushi Yabushita*, Takayoshi Kobayashi, Pei Feng Chen, Keng S. Liang

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Ultrafast dynamics of endothelial nitric oxide synthase (eNOS) oxygenase domain was studied by transient absorption spectroscopy pumping at Soret band. The broadband visible probe spectrum has visualized the relaxation dynamics from the Soret band to Q-band and charge transfer (CT) band. Supported by two-dimensional correlation spectroscopy, global fitting analysis has successfully concluded the relaxation dynamics from the Soret band to be (1) electronic transition to Q-band (0.16 ps), (2) ligand dissociation and CT (0.94 ps), (3) relaxation of the CT state (4.0 ps), and (4) ligand rebinding (59 ps).

Original languageEnglish
Pages (from-to)619-624
Number of pages6
JournalChemical Physics Letters
Volume683
DOIs
StatePublished - 1 Sep 2017

Keywords

  • Nitric oxide synthase
  • Two dimensional correlation spectroscopy
  • Ultrafast transient absorption spectroscopy

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