Ultra sensitive detection of eneterovirus 71 by modified electrochemical impedance spectroscopy

Shin Hua Tseng, Dion T. Tseng, Tzu Cheng Lee, Tsai Mu Cheng, Jyh Yuan Yang, Ruo Yu Hsieh, Chuan Mei Tsai, Chia-Ching Chang*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (∼sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.

Original languageEnglish
Title of host publicationProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
Pages27-28
Number of pages2
StatePublished - 4 Aug 2010
Event1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, United States
Duration: 7 Feb 201010 Feb 2010

Publication series

NameProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010

Conference

Conference1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
CountryUnited States
CityHouston, TX
Period7/02/1010/02/10

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