Tyrosine-kinase expression profiles in human gastric cancer cell lines and their modulations with retinoic acids

H. W. Kao, H. C. Chen, C. W. Wu, W. C. Lin*

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Many protein tyrosine kinases are key regulators involved in cellular growth, differentiation, development, apoptosis and signal transduction pathways. Obtaining a comprehensive tyrosine-kinase expression profile in tumour cells is essential to learning more about their oncogenic potentials and responses to various chemotherapeutic reagents - such as retinoic acid, which has been shown to suppress the growth of gastric cancer cells and modulate gene expression. Expression of tyrosine kinases in retionic acid-treated cancer cells was investigated by reverse trancriptase-polymerase chain reaction (RT-PCR) and a novel restriction analysis of gene expression (RAGE) display technique. We first established comprehensive tyrosine-kinase profiles in different human gastric cancer cell lines. In cells treated with 9-cis-retinoic acid or all-trans-retinoic acid, we found that two PTKs (Eph and Hek5) appeared to be upregulated. In the present study, we demonstrate an efficient and simple RAGE approach for examining tyrosine kinases' expression in tumour cells and their alterations following drug treatments.

Original languageEnglish
Pages (from-to)1058-1064
Number of pages7
JournalBritish Journal of Cancer
Volume88
Issue number7
DOIs
StatePublished - 7 Apr 2003

Keywords

  • Gene expression profiles
  • Protein-tyrosine kinase
  • Retinoic acid

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