Abstract
Oxidosqualene-lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants' construction of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3β-ol, lanosterol, and parkeol. Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps.
Original language | English |
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Pages (from-to) | 1319-1322 |
Number of pages | 4 |
Journal | Organic Letters |
Volume | 8 |
Issue number | 7 |
DOIs | |
State | Published - 30 Mar 2006 |