Tryptophan 232 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences rearrangement and deprotonation but not cyclization reactions

Tung-Kung Wu*, Mei Ting Yu, Yuan Ting Liu, Cheng Hsian Chang, Hsing Ju Wang, Wei-Guang Diau

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Oxidosqualene-lanosterol cyclases convert oxidosqualene to lanosterol in yeast and mammals. Site-saturated mutants' construction of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase, at Trp232 exchanges against proteinogenic amino acids, and product profiles are shown. All mutants, except Lys and Arg, produced protosta-12,24-dien-3β-ol, lanosterol, and parkeol. Overall, Trp232 plays a catalytic role in the influence of rearrangement process and determination of deprotonation position but does not involve intervention in the cyclization steps.

Original languageEnglish
Pages (from-to)1319-1322
Number of pages4
JournalOrganic Letters
Volume8
Issue number7
DOIs
StatePublished - 30 Mar 2006

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