Trapping performance of Fe 3 O 4 Αl 2 O 3 and Fe 3 O 4 ΤiO 2 magnetic nanoparticles in the selective enrichment of phosphopeptides from human serum

Cheng Tai Chen, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

We have previously demonstrated that both alumina-coated iron oxide magnetic nanoparticles (Fe 3 O 4 Αl 2 O 3 ) and titania-coated iron oxide magnetic nanoparticles (Fe 3 O 4 ΤiO 2 ) are effective affinity probes for phosphopeptides from the tryptic digests of phosphoproteins. The major advantage of these two types of affinity probe is the ease of isolating the affinity probe-target species conjugates from complex samples based on their magnetic property. Only a short time is required to enrich phosphorylated species in sufficient amounts for MALDI MS analysis by vigorously mixing the suspension of the sample and affinity nanoparticles through pipeting in and out of a sample vial for 30 sec. We herein evaluate the trapping performance of Fe 3 O 4 Αl 2 O 3 and Fe 3 O 4 ΤiO 2 magnetic nanoparticles as the affinity probes to selectively enrich phosphopeptides from human serum under the same experimental condition. The peaks derived from fibrinopeptide A containing a phosphorylated serine dominate the matrix-assisted laser desorption/ionization (MALDI) mass spectra of the sample obtained after using the affinity probes to selectively enrich their target species from human serum. Furthermore, only phosphorylated fibrinopeptide A residues, which appeared in the MALDI mass spectrum, as Fe 3 O 4 Αl 2 O 3 magnetic nanoparticles are employed as the affinity probes for the serum sample. However, non-phosphopeptides also appear in the MALDI mass spectrum when using Fe 3 O 4 ΤiO 2 magnetic nanoparticles as the affinity probes for the same serum sample. The results suggest that the specificity of Fe 3 O 4 Αl 2 O 3 magnetic nanoparticles for phosphopeptide is superior to that of Fe 3 O 4 ΤiO 2 magnetic nanoparticles.

Original languageAmerican English
Pages (from-to)73-79
Number of pages7
JournalJournal of Biomedical Nanotechnology
Volume4
Issue number1
DOIs
StatePublished - Dec 2008

Keywords

  • Alumina
  • Fibrinopeptide A
  • Magnetic nanoparticles
  • Phosphopeptides
  • Serum
  • Titania

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