Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry

An Cheng Liu, Liang Yu Chen, Chung Fan Chiou, Hung Ju Richard Su, Yu Chia Chan, Pei Hsuan Tasi, Shean-Jen Chen, Wen Yih Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

This investigation presents measurements of isothermal ssDNA oligomer hybridization enthalpy at various DNA length, GC contents, temperatures, and salt concentrations by isothermal titration calorimetry (ITC) at temperatures below the melting temperature of the DNA. The study aimed to reveal the hybridization mechanism of ssDNA for gene chip applications. The role of hydrogen bonds between complementary bases, the stacking forces between bases, the electrostatic repulsion between strands, the dehydration and conformational rearrangement of ssDNA in hybridization are estimated from the interaction enthalpy.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalThermochimica Acta
Volume433
Issue number1-2
DOIs
StatePublished - 1 Aug 2005

Keywords

  • DNA hybridization
  • Enthalpy
  • Gene chip
  • Isothermal titration calorimetry

Fingerprint Dive into the research topics of 'Thermodynamics and mechanism of ssDNA hybridization below the melting temperature by isothermal titration calorimetry'. Together they form a unique fingerprint.

Cite this