The XopE2 effector protein of Xanthomonas campestris pv. vesicatoria is involved in virulence and in the suppression of the hypersensitive response

Rong Hwa Lin, Chih Wen Peng, Yuan Chuen Lin, Hwei-Ling Peng, Hsiou Chen Huang

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1 Scopus citations

Abstract

Pathogenicity of Xanthomonas campestris pv. vesicatoria (Xcv) causing bacterial spot disease on tomato (Lycopersicon spp.) and pepper (Capsicum spp.) requires the type III secretion system (T3SS) and T3SS effectors. In this study, we employed the AFLP technique to investigate the diversity of X. campestris p v. vesicatoria isolated in Taiwan, and consequently a XopE2 homologue was identifed in all fourteen Xcv strains that have been classifed into two groups. Phylogenic analysis of XopE2 amino acid sequences indicated that XopE2 of Xcv Xvt122 (group A) has a closer genetic distance to XopE2 of Xcv 85-10 than to that of Xcv Xvt45 (group B). Interestingly, although it was suggested that Xvt45 contains duplicated xopE2 genes, one being located on the genome and the other located on a large plasmid, a single copy deletion of xopE2 within the genome caused a substantial reduction in virulence, but no effect of xopE2 mutation on virulence of Xcv 85-10 and Xvt122 was observed. Furthermore, our results revealed that XopE2 of Xcv Xvt122 or Xcv Xvt45 was able to suppress HR in a T3SS-dependent manner and the heterologously-expressed XopE2 was suffcient to modulate the virulence on susceptible tomato plants. Their biological functions are not dependent on the consensus catalytic triad (159th cysteine) and thiol-protease His residue (47th histidine) of XopE2.

Original languageEnglish
Pages (from-to)55-72
Number of pages18
JournalBotanical Studies
Volume52
Issue number1
StatePublished - 1 Jan 2011

Keywords

  • Amplified restriction fragment length polymorphism
  • Bacterial spot
  • Hypersensitive response
  • Type III secretion system
  • XopE2 effector

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