The treatment of propofol induced the TGF-β1 expression in human endothelial cells to suppress endocytosis activities of monocytes

Chi Han Li, Ru Ping Lee, Yu Ling Lin, Chen Si Lin, Bang Gee Hsu, Feng Jen Tseng, Yu Cheng Chen, Kuang-Wen Liao*, Fwu Lin Yang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Propofol anesthesia and sedation are known to downregulate the functions of many hematopoietic cells, such as macrophages and neutrophils, in vivo. However, the effects of propofol on secretion of the regulatory cytokine transforming growth factor β1 (TGF-β1) in vivo are unknown. In this study, the effects of propofol on TGF-β1 expression in human peripheral blood mononuclear cells, umbilical vein endothelial cells (HUVECs), lymphocytes (Jurkat) and monocytes (THP-1) were tested. Moreover, these sera were also tested for regulatory activity on monocyte endocytosis with or without treatment with the TGF-β1 pathway inhibitor SB431542. Propofol raised levels of both total and activated TGF-β1 in propofol-treated patient sera after surgical operations. Furthermore, propofol induced secretion of latent TGF-β1 in HUVEC cells and enhanced TGF-β1 activation in THP-1 and Jurkat cells in vitro. Additionally, sera from propofol-treated patients suppressed monocyte endocytosis ex vivo, an effect that was abrogated by the TGF-β1 pathway inhibitor SB431542.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalCytokine
Volume52
Issue number3
DOIs
StatePublished - 13 Sep 2010

Keywords

  • Endothelial cells
  • Immunosuppression
  • Monocytes
  • Propofol
  • TGF-β1

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