The effect of biomimetic nanostructured cell matrixes on neural behaviors

Yen Pei Lu, Ming Yu Lin, Ting Gang Chen, Min An Tsai, Peichen Yu, Yu Cheng Ou, Fu Han Ho*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In order to investigate the neural behaviors on nanostructure, we proposed the nanostructured substrates coated with different extracellular matrix proteins for culturing neuron cells and induced the neural differentiation by nerve growth factor (NGF). The artificial nanostructured surface is the nanorods fabricated on the silicon surface by the chemical etching method. Three kinds of substrates: cover glass slide, flat silicon wafer, and nanostructured wafer were treated with oxygen plasma to increase hydrophilic property, and coated with the 1% ECM gel, laminin-1 peptides, and IKVAV peptide respectively. Neuron PC12 cells, were cultured on the processed substrates and were induced to differentiate neurites with NGF. Distribution of cytoskeleton, cellular attachment, body morphology, and neuritogenesis were studied by scanning electron microscopy (SEM) and immunofluorescence assay (IFA). The results reveal that different substrates will influence the behavior of neuron differentiation.

Original languageEnglish
Title of host publication7th Asian-Australasian Conference on Composite Materials 2010, ACCM 2010
PublisherACCM-7 Organizing Committee
Pages797-799
Number of pages3
ISBN (Print)9781632660756
StatePublished - 1 Jan 2010
Event7th Asian-Australasian Conference on Composite Materials 2010, ACCM 2010 - Taipei, Taiwan
Duration: 15 Nov 201018 Nov 2010

Publication series

Name7th Asian-Australasian Conference on Composite Materials 2010, ACCM 2010
Volume2

Conference

Conference7th Asian-Australasian Conference on Composite Materials 2010, ACCM 2010
CountryTaiwan
CityTaipei
Period15/11/1018/11/10

Keywords

  • Biomimetic
  • Nanostructure
  • Neural behaviors

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