The design of a novel complementary metal oxide semiconductor detection system for biochemical luminescence

Ude Lu, Ben C.P. Hu, Yu Chuan Shih, Chung-Yu Wu, Yuh-Shyong Yang*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We designed a complementary metal oxide semiconductor (CMOS) chip with accompanied accessories as a system for the detections and quantifications of biochemical luminescence. This is the first of such instruments that has been reported. The semiconductor chip was manufactured through a 0.25μm CMOS standard process. A current mirror was designed in integrated circuit (IC) to amplify the signal current that was induced by chemiluminescence. Horseradish peroxidase (HRP)-luminol-H2O2 system was used as an example to constitute a useful platform for coupling to chemiluminescence reactions which produce H2O2. Glucose-glucose oxidase (GOD) reaction was coupled with HRP-luminol-H2O2 reaction to demonstrate the ability of the novel CMOS base instrument for quantifying the biological luminescence of a variety of valuable clinical assays. Our results illustrated that the combination of the specifically designed CMOS IC and commercially available electronic devices established a simple and useful bioanalytical tool.

Original languageEnglish
Pages (from-to)1185-1191
Number of pages7
JournalBiosensors and Bioelectronics
Volume19
Issue number10
DOIs
StatePublished - 15 May 2004

Keywords

  • Biochemical analysis
  • Bioluminescence
  • CMOS
  • Enzyme chip
  • IC design

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