The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death

Kai Wen Hsu, Chien Yu Huang, Ka Wai Tam, Chun-Yu Lin, Li Chi Huang, Ching Ling Lin, Wen Shyang Hsieh, Wei Ming Chi, Yu Jia Chang, Po Li Wei, Shou Tung Chen*, Chia Hwa Lee

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic.

Original languageEnglish
Article number452
JournalInternational journal of molecular sciences
Volume19
Issue number2
DOIs
StatePublished - 2 Feb 2018

Keywords

  • HDACi
  • Live cell non-invasive apoptosis detection sensor (NIADS)
  • Triple negative breast cancer (TNBC)

Fingerprint Dive into the research topics of 'The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death'. Together they form a unique fingerprint.

  • Cite this

    Hsu, K. W., Huang, C. Y., Tam, K. W., Lin, C-Y., Huang, L. C., Lin, C. L., Hsieh, W. S., Chi, W. M., Chang, Y. J., Wei, P. L., Chen, S. T., & Lee, C. H. (2018). The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death. International journal of molecular sciences, 19(2), [452]. https://doi.org/10.3390/ijms19020452